Stability-indicating liquid chromatographic method for valacyclovir
نویسندگان
چکیده
A novel stability-indicating high-performance liquid chromatographic assay method was developed and validated for valacyclovir in the presence of degradation products generated from forced decomposition studies. A Hypersil ODS C-18 (250 x 4.6 mm, packed with 5 micron) column in an isocratic mode with the mobile phase acetonitrile: phosphate buffer (pH3.6) (50:50%v/v) was used. The flow rate was 0.8ml/ min and the detection was done at 252 nm. The retention time was found to be 2.850min. The linearity range was found to be 0.5 200 mg/ml. The present method is also useful for the estimation of valacyclovir in pharmaceutical dosage forms. The proposed method was subsequently validated. The method even proved to be affective on application to a stressed marketed formulation.
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